FIGURE

Fig. 1

ID

ZDB-FIG-181206-26

Publication

Barrodia et al., 2018 - EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

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Fig. 1

Expression of efhc2 mRNA during zebrafish development. RT-PCR and whole embryo mRNA in situ hybridization (WISH) was carried out on different stages of zebrafish development. a RT-PCR analysis of efhc2 mRNA expression during zebrafish development. ef1α was used as loading control. b Ubiquitous expression of efhc2 at 6 hpf. c efhc2 expression in kupffer’s vesicle (KV) at 9 hpf. d Expression in the intermediate mesoderm (IM), notochord (NC) and otic vesicle (O) at 12 hpf. e Expression of efhc2 in olfactory placode (OP), pronephros (P), epiphysis (E), notochord (NC), otic vesicle (O) and in tailbud (TB) at 24 hpf. f Magnified image of zebrafish trunk show strong expression of efhc2 in PST and DE and low expression in PCT, DL and PD. g Dorsal view of 24 hpf embryos showing its expression in pronephros. h, i Two colour WISH of pdzk1 and efhc2. J Section through trunk showing efhc2 expression in pronephros (P). k, l Expression of efhc2 in epiphysis, pronephros, notochord, otic vesicle and in tail bud at 36 and 48 hpf. (M) Expression of efhc2 in neuromast cells and olfactory placode at 72 hpf

Expression Data

Gene:	efhc2
Fish:	WT
Anatomical Terms:	epiphysis forerunner cell group intermediate mesoderm neuromast notochord olfactory placode otic placode otic vesicle peripheral olfactory organ post-vent region pronephros tail bud whole organism (all 13)
Stage Range:	2-cell to Protruding-mouth

Expression Detail

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
efhc2	WT	standard conditions	2-cell to 1k-cell	Not Detected	whole organism	RTPCR
efhc2	WT	standard conditions	Shield		whole organism	ISH
efhc2	WT	standard conditions	Shield to Protruding-mouth		whole organism	RTPCR
efhc2	WT	standard conditions	90%-epiboly		forerunner cell group	ISH
efhc2	WT	standard conditions	5-9 somites		intermediate mesoderm	ISH
efhc2	WT	standard conditions	5-9 somites		notochord	ISH
efhc2	WT	standard conditions	5-9 somites		otic placode	ISH
efhc2	WT	standard conditions	Prim-5		epiphysis	ISH
efhc2	WT	standard conditions	Prim-5		notochord	ISH
efhc2	WT	standard conditions	Prim-5		olfactory placode	ISH

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Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Biosci.