FIGURE

Fig. 5

ID

ZDB-FIG-181127-21

Publication

Sun et al., 2018 - Mga Modulates Bmpr1a Activity by Antagonizing Bs69 in Zebrafish

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Fig. 5

Mga binds to Bs69 and disrupts the Bs69–Bmpr1a interaction. (A) Increasing dose of pCS2-Mga (1, 2, 3 μg) along with 1 μg pCS2-HA-Bs69, 1 μg pCS2-Bmpr1a-FLAG were transiently co-transfected into 293T cells, and co-immunoprecipitation of HA-Bs69 and Bmpr1a-FLAG was analyzed. (B) Increasing dose of pCS2-Bmpr1a-FLAG (1, 2, 3 μg) along with 1 μg pCS2-HA-Bs69, 1 μg pCS2-Mga were transiently co-transfected into 293T cells, and co-immunoprecipitation of HA-Bs69 and Mga was analyzed. (C) One-cell-stage wild-type embryos injected with 50 pg mRNA encoding HA-Bs69 or mixture of mRNAs encoding both HA-Bs69 and mouse MGA, or co-injected with 50 pg mRNA encoding HA-Bs69 with 100ng pCS2-FLAG-Mga or pCS2-FLAG-MgaΔ. Immunoblot analysis of pSmad1/5 levels was performed with lysates from the injected embryos at 8 hpf. (D) Relative quantification of pSmad1/5 levels of panel C. (E) One-cell-stage wild-type embryos injected with 50 pg mRNA encoding HA-Bs69 or mixture of mRNAs encoding both HA-Bs69 and mouse or zebrafish MGAs, and the ventral tail fin phenotypes of these embryos at 48 hpf were shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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