FIGURE

Fig. 5

ID

ZDB-FIG-181108-15

Publication

Ranski et al., 2018 - Characterization of retinal regeneration in adult zebrafish following multiple rounds of phototoxic lesion

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Fig. 5

Following multiple rounds of regeneration, retinas exhibit abnormal localization of inner retinal neurons.(A–F) Retinal sections collected at 28 dpL immunolabeled with anti-GFAP and anti-HuC/D to show Müller glia and inner retinal neurons, respectively, in untreated control (No Light Treatment), experimental control (1×Light Treatment) and experimental retinas (1×Light Treatment). Nuclei are stained in blue with TO-PRO-3. (A–C) Müller glia are immunolabeled with anti-GFAP. (D–F) Amacrine and ganglion cells are immunolabeled with anti-HuC/D. Mis-localization of HuC/D-positive cells were observed in the inner plexiform layer (IPL; arrowhead) and outer retina (arrow). (G) Quantification of average numbers of HuC/D-positive cells found in the IPL and outer retina (n = 5 per group). Cells were counted over a linear distance of 300 μm on the central dorsal retina. Asterisk indicates significantly different from 0 h control; Psi symbol indicates significantly different from 1×retinas (p < 0.05). Scale bar represents 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	light ablation: photoreceptor cell
Observed In:	retinal inner nuclear layer amacrine cell retinal inner nuclear layer retinal ganglion cell retinal inner plexiform layer amacrine cell retinal inner plexiform layer retinal ganglion cell retinal outer plexiform layer amacrine cell retinal outer plexiform layer retinal ganglion cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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