FIGURE

Fig. 3

ID

ZDB-FIG-181023-2

Publication

Yamaguchi et al., 2018 - Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

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Fig. 3

Cryo-ET revealed structural defects of axonemal dyneins in mutant spermatozoa.

(A) DMT structure of native zebrafish sperm. Left: side view. Right: base-to-tip view. A-tub and B-tub: A- and B-tubule of DMT, respectively. (B) Diagram of the DMT structure of zebrafish sperm. f IC/LC means IAD f intermediate chain and light chain complex. (C) DMT structure of human respiratory cilia (EMD-5950; Lin et al., 2014). (D) DMT structure of Chlamydomonas flagella (DMT 2–8 averaged; EMD-2132; Bui et al., 2012). Red arrow indicates a linker between N-DRC and OAD. (E) Diagram of the DMT structure of Chlamydomonas flagella. (F–K) DMT structures of PIH gene mutant spermatozoa. For pih1d2^-/-;ktu^-/-, J (+OAD) and K (-OAD) represent subtomograms of axonemes with or without OADs, respectively. Red circles indicate the defects of axonemal dyneins. Green, OADs; red, IAD f; orange, IAD a; yellow, IAD b; light-green, IAD c; cyan, IAD e; indigo, IAD g; violet, IAD d; blue, RSs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnaaf2^ja104/ja104 (TL) dnaaf6^ja105/ja105 (TL) pih1d1^ja102/ja102 pih1d2^ja103/ja103 pih1d2^ja103/ja103; dnaaf2^ja104/ja104
Observed In:	sperm axonemal dynein complex sperm inner dynein arm sperm outer dynein arm
Stage:	Adult

Phenotype Detail

Acknowledgments

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