FIGURE

Fig. 2

ID

ZDB-FIG-181011-8

Publication

Lahvic et al., 2018 - Specific oxylipins enhance vertebrate hematopoiesis via the receptor GPR132

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Fig. 2

gpr132b is required for EET’s enhancement of zebrafish developmental hematopoiesis. (A) Single-cell zebrafish embryos were injected with 4–6 ng gpr132b MO or control MO or were uninjected, and then treated with DMSO or 5 μM 11,12-EET beginning at 24 hpf. Embryos were fixed at 36 hpf and stained for runx1 expression. (Scale bar, 100 μm.) (B) Embryos were scored as having high or normal runx1 expression in the AGM, and high (present) or normal (absent) runx1 expression in the tail mesoderm. Graph presents summary of several experiments, total number of embryos at base of each column. Two-tailed Fisher’s exact test. Ctrl includes control MO injected and uninjected embryos.

Expression Data

Gene:	runx1
Fish:	WT WT + MO2-gpr132b
Condition:	chemical treatment by environment: 11,12-EET
Knockdown Reagent:	MO2-gpr132b
Anatomical Terms:	caudal fin ventral wall of dorsal aorta
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO2-gpr132b
Condition:	chemical treatment by environment: 11,12-EET
Knockdown Reagent:	MO2-gpr132b
Observed In:	caudal fin ventral wall of dorsal aorta
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA