FIGURE

Fig. S8

ID

ZDB-FIG-180924-13

Publication

Fauquier et al., 2018 - CBP and P300 regulate distinct gene networks required for human primary myoblast differentiation and muscle integrity

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Fig. S8

P300/CBP histone Acetyltransferase activity inhibition does not induce apotosis in muscle cells when fivers detach in zebrafish

(a) Confocal projections of embryos treated with either DMSO (vehicle), 2μm or 3.5 μM C646 at 10 hpf for 24 h, fixed and immunostained against caspase 3 and imaged at 6 dpf (6D). Topro3 was used to stain the nuclei. (b) Relative mRNA expressions of bcl2,bax and puma were determined by RT-qPCR in 3 dpf embryos treated with either DMSO, or 2 μm or 3.5 μm C646 from 10 hpf for 24 h. Error bars represent S.D of 3 independent experiments. Statistical significance is relative to control and was calculated with an unpaired t-test. ns: not significant. A minimum of 12 embryos were analysed per condition.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i104Tg
Condition:	chemical treatment by environment: C646
Observed In:	muscle cell apoptotic process
Stage:	Day 6

Phenotype Detail

Acknowledgments

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