FIGURE

Fig. 2

ID

ZDB-FIG-180913-53

Publication

Asakawa et al., 2018 - Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus

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Fig. 2

Soma Size Topography of mnr2b-ABNs

(A) Left abducens nerve terminal of Tg[mnr2b-hs:Gal4]; Tg[UAS:GFP] larva at 5 dpf.

(B–E) Axon terminals (orange) and somata (blue) of single abducens motor neurons.

(F) Two neighboring abducens motor neurons on the dorsal side of r5, possessing either an en grappe-type (left arrowhead) or en plaque-type (right arrowhead) terminal, respectively.

(G) The soma size of the dorsal and ventral cells in r5 and r6. N = 40 cells from 22 animals. ^∗p < 0.05 (t test).

(H and I) The lateral (H) and dorsal (I) views of an mnr2b-ABNs in the Tg[mnr2b-hs:Gal4]; Tg[UAS:Kaede] embryo at 44 hpf.

(J) Birth-order labeling of mnr2b-ABNs and possible segregation patterns of early-born neurons along the dorsoventral axis (magenta in I, II, and III). Green fluorescence of Kaede that appears after photoconversion is indicated as post-Kaede.

(K and L) Dorsal (K) and ventral (L) sections of r5 and r6 in the photoconverted Tg[mnr2b-hs:Gal4]; Tg[UAS:Kaede] larvae at 72 hpf.

Scale bars indicate 50 μm in (A)–(F) and 20 μm in (H), (I), (K), and (L).

Expression Data

Genes:	EGFP Kaede
Fish:	nkmnr2bhsga14Tg; nkuasgfp1aTg nkmnr2bhsga14Tg; s1999tTg
Anatomical Terms:	abducens motor nucleus axon abducens motor nucleus neuronal cell body rhombomere 5 abducens motor nucleus rhombomere 6 abducens motor nucleus
Stage Range:	High-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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