FIGURE

Fig. 6

ID

ZDB-FIG-180912-57

Publication

Neelathi et al., 2018 - Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.

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Fig. 6

Delayed Wnt Fgf signaling dynamics in stalled primordia. (A) In control embryos, the primordium (pLLp) migrates and separates from the pLL ganglion (pLLg) by 22 hpf and lef1 expression is restricted to the leading domain. (B,C) With migration inhibited in cxcl12a morphants and PP1-treated embryos, the primordium remains juxtaposed with the pLLg and lef1 expression remains broad. (D) pea3 expression at the trailing end of the primordium in controls at 22 hpf. (E,F) The pea3 expression domain is still at the trailing end of the prospective primordium in the absence of migration; however, the pea3 domain is in the middle of a broad lef1-expressing domain, which now represents the juxtaposed primordium and the pLLg. (G-I) snail1b expression in control, cxcl12a morphant and PP1-treated primordia at 22 hpf. OV, otic vesicle. n=25 for each of two independent experiments. Dotted lines encircle pLL primordia.

Expression Data

Genes:	etv4 lef1 snai1b
Fish:	zf106Tg zf106Tg + MO4-cxcl12a
Condition:	chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Knockdown Reagent:	MO4-cxcl12a
Anatomical Terms:	posterior lateral line primordium posterior lateral line primordium anterior region posterior lateral line primordium central region posterior lateral line primordium posterior region
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg zf106Tg + MO4-cxcl12a
Condition:	chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Knockdown Reagent:	MO4-cxcl12a
Observed In:	posterior lateral line ganglion posterior lateral line placode posterior lateral line primordium posterior lateral line primordium central region posterior lateral line primordium posterior lateral line neuromast primordium migration
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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