Fig. 2

Altered Microglia Distribution and Numbers in the Adult csf1r Mutant Brain

(A) Representative images of microglia in WT, csf1ra^−/−, csf1ra^−/−;b^+/−, and csf1r^DM brain sections from zebrafish aged 5 months post fertilization (mpf), stained with antibody against L-plastin (n = 3 zebrafish/group).

(B) Microglia in (a) the ventral part of the optic tectum and (b) the dorsolateral part of the optic tectum of WT, csf1ra^−/−, csf1ra^−/−;b^+/−, and csf1r^DM.

(C) Representative images of microglia in WT, csf1ra^−/−, and csf1rb^−/− brain sections from 15 mpf zebrafish stained with antibody against L-plastin (n = 3 zebrafish/group).

Microglia were quantified in 3 areas (2.5 × 10⁶ μm³) per brain region per animal. Error bars represent SD. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (one-way ANOVA, Bonferroni multiple testing correction). Scale bars in (A) and (B), top, indicate 200 μm; in (A) and (B), bottom, and in (C), scale bars indicate 50 μm.