FIGURE

Fig. 2

ID

ZDB-FIG-180907-6

Publication

Powell et al., 2017 - Chemokine Signaling and the Regulation of Bidirectional Leukocyte Migration in Interstitial Tissues

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Fig. 2

Cxcr2 Is Required for Neutrophil Response to Cxcl8a and Pseudomonas aeruginosa Infection

(A) Representative imaging of the otic vesicle (white dotted outline) 2 hr after injection with supernatant from HEK cells overexpressing zebrafish Cxcl8a or control supernatant. Supernatant was injected into the otic vesicle of 3 dpf Tg(mpx:Dendra) WT or Cxcr2−/− larvae (neutrophils in green).

(B) Number of neutrophils in the otic vesicle of Cxcl8a or control supernatant injected larvae (n = 59 WT and 58 mutant [Ctrl] and 56 WT and 54 mutant [zCxcl8a]).

(D) Representative imaging of the otic vesicle (white dotted outline) 2 hr after injection with P. aeruginosa in WT or Cxcr2 mutant Tg(mpx:Dendra) larvae.

(E) Number of neutrophils in the otic vesicle of P. aeruginosa or vehicle-injected larvae (n = 62 WT and 73 mutant [PBS] and 76 WT and 76 mutant [P. aeruginosa]).

(F) Neutrophil fold change response to P. aeruginosa over PBS control. ^∗p < 0.05.

Error bars represent SE.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cxcr2^uwm35/uwm35; uwm4Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa
Observed In:	neutrophil chemotaxis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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