FIGURE

Fig. S8

ID

ZDB-FIG-180906-54

Publication

Ouyang et al., 2017 - Hyaluronic acid synthesis is required for zebrafish tail fin regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

Wnt pathway activity is not upregulated during larval tail regeneration or modulated by 4-MU or GSK3 inhibitors.

Whole-mount analysis of GFP expression in both wild type (A-A’) and Tg(top:GFP) embryos (B-E and B’-E’) at 1 dpa (3 dpf), following treatments with DMSO, 150 μM 4-MU, 100 nM BIO, or 50 μM SB216763 from 2 to 3 dpf. The Tg(top:GFP) embryos express a destabilized form of GFP under control of a minimal cFos promoter with four TCF/LEF binding sites, providing a dynamic readout of Wnt pathway state. At least 30 larvae were analyzed for each experimental condition, and phenotypic descriptions were based on a penetrance of > 80%. Scale bar: 300 μM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One