FIGURE

Fig. 3

ID

ZDB-FIG-180829-12

Publication

Liu et al., 2018 - CRMP2 and CRMP4 Are Differentially Required for Axon Guidance and Growth in Zebrafish Retinal Neurons

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Fig. 3

Knocking down CRMP4 causes ipsilateral misprojections of retinal axons. Morpholino was injected into 1-2 cell stage embryos and retinal axons were labeled with DiI or DiD at 4 dpf. (a) A representative image demonstrating that, in control MO-treated zebrafish larvae, all retinal axons cross the midline and project into the opposite side of the tectum. (b, c) Representative images of retinal axon guidance errors in CRMP4 MO-treated larvae. A part or all of the retinal axons fail to cross the midline and misproject into the ipsilateral tectum (arrows). Note that although the axons misproject ipsilaterally, they still follow the normal optic tract and arborize into the tectum. (d) The ipsilateral misprojections of retinal axons caused by CRMP4 MOs are dose dependent. The doses of MOs are labeled under each column. The numbers in parentheses above each column indicate the amount of eyes. Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dpysl3
Knockdown Reagent:	MO1-dpysl3
Observed In:	retinal neural layer axon retinal neural layer axon guidance
Stage:	Day 4

Phenotype Detail

Acknowledgments

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