FIGURE

Fig. 5

ID

ZDB-FIG-180828-64

Publication

Tsai et al., 2018 - Small molecule inhibition of RAS/MAPK signaling ameliorates developmental pathologies of Kabuki Syndrome

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Fig. 5

dmDf ameliorates cell proliferation defects in the brain caused by loss of kmt2d or rap1. (a) Depletion of kmtd2 and kdm6a through morpholino injection leads to a significant reduction in the number of proliferating cells in the brain, visualized with immunostaining for phospho-histone H3 (pHH3) positive cells, in 2 dpf embryos. Treatment with dmDf significantly increases pHH3+ proliferating cells. (b) kmt2d-CRISPR and rap1-CRISPR embryos also exhibit a significant reduction in pHH3+ cells in the brain that can be rescued by treatment with dmDf. *p < 0.05; **p < 0.01; ***p < 0.001. Error bars show SEM (standard error of the mean).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO2-kdm6a EKW + MO5-kmt2d EKW + CRISPR1-rap1ab + CRISPR1-rap1b
Condition:	chemical treatment by environment: B-Raf inhibitor
Knockdown Reagents:	CRISPR1-rap1ab CRISPR1-rap1b MO2-kdm6a MO5-kmt2d
Observed In:	brain cell population proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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