FIGURE

Fig. S2

ID

ZDB-FIG-180827-57

Publication

Harjula et al., 2018 - Interleukin 10 mutant zebrafish have an enhanced interferon gamma response and improved survival against a Mycobacterium marinum infection

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Fig. S2

Unchallenged il10^e46/e46 zebrafish have similar expression profiles of lymphocyte markers and cytokines as WT control fish in the intestine. (a) The relative expression of il10, selected proinflammatory cytokines (il1b, tnfa, tnfb), Th cell cytokines (ifng1 and il4), T cell markers (cd4-1 and cd8a), a B cell marker (IgM) as well as Cd4+ lymphocyte transcription factors (tbx21, gata3, foxp3a) in il10^e46/e46 mutant zebrafish (n = 9) and WT controls (n = 13) in intestine were quantified with qPCR and are represented as scatter dot plot and median. Note the different scales of the y axes and the divided y axis. Gene expressions were normalized to the expression of eef1a1l1. A two-tailed Mann-Whitney test was used for the statistical comparison of differences between il10^e46/e46 zebrafish and WT controls. (b) The visual appearance of il10^e46/e46 mutant and WT control zebrafish intestine was studied by microscopy (n = 3 in both groups). A representative image from each group is shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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