Fig. 5
- ID
- ZDB-FIG-180817-29
- Publication
- Hofsteen et al., 2018 - ALPK2 Promotes Cardiogenesis in Zebrafish and Human Pluripotent Stem Cells
- Other Figures
- All Figure Page
- Back to All Figure Page
ALPK2 Inhibits WNT/β-Catenin Signaling to Promote Cardiogenesis (A) Transcripts abundance of ALPK2 in control and ALPK2 shRNA-transfected cardiac progenitor cells (CPCs) derived from human embryonic stem cells (hESCs). (B) Flow cytometric plot and graphs quantifying venus expression driven by β-catenin activated reporter (BAR) in live CPCs following control or ALPK2 shRNA transfection as hESCs. (C) Western blot protein expression analysis of WNT/β-catenin signaling modulators in wild-type and CRISPR/Cas9-mediated ALPK2 mutant hESC-derived CPCs (ALPK2Δ10) treated with a small molecule direct tankyrase inhibitor XAV939 (1 μM). (D) Western blotting for the WNT/β-catenin signaling target LEF1 in wild-type and ALPK2Δ10 cells with or without the addition of XAV939 (0 or 1 μM). (E) TNNT2 flow cytometry quantification of day 14 cardiomyocytes derived from wild-type and ALPK2Δ10 hESCs treated with XAV939 (0, 1, 5 μM). (F) Representative images of heat shocked (hs) wild-type and hsWnt8b transgenic zebrafish carrying a transgene for the epicardium (tcf21:DsRed) at 96 hpf. Fish were counterstained with an activated leukocyte cell adhesion molecule (ALCAM; magenta). N = 3–5 biological replicates, and data are displayed as mean ± SEM. * = p ≤ 0.05. See also Figure S4. |
| Fish: | |
|---|---|
| Observed In: | |
| Stage: | Day 4 |