Fig. 1-S1
Validation of the meis2b reporter line. (a) High magnification of Figure 1g showing the asymmetric expression of meis2b in the atrium of the adult zebrafish heart (red arrow, meis2b + atrial domain; black arrow, meis2b- atrial domain). (b) Schematic of the TgBAC(meis2b:GAL4FF);Tg(5xUAS:EGFP) meis2b reporter system. (c–f) Comparison of meis2b expression as detected by whole-mount in situ hybridization and Tg(meis2b-reporter) expression at 24 and 48 hpf. Note: in situ hybridization staining reaction was stopped before spinal CNS expression was fully detected in order to avoid overstaining of the head region. Scale bars: 500 µm. |