Fig. 3

ism1 knockdown decreases neutrophils and macrophages in 48 hpf zebrafish.

mpx:GFP single-cell-stage embryos were injected with 7 ng ism1 MO (MO; squares), or 7 ng ism1 MO and 17.88 ng of ism1 mRNA (Rescue; triangles); uninjected embryos served as controls (circles). (A) Developing 48 hpf zebrafish were visualized, and individual mpx:GFP⁺ cells were enumerated; representative fish shown for reference. (B) Each data point represents total amount of mpx:GFP⁺ cells present in one zebrafish. (C) qRT-PCR was performed comparing transcript levels of csf3r (left) and csf1r (right) in ism1 MO injected (MO) and uninjected controls (control) at 48 hpf. Bars represent the mean, and error bars represent standard deviation. A two-tailed unpaired Student’s T test was performed to determine statistical significance. * denotes p<0.05, ** denotes p<0.0001, N.S indicates no significance.