FIGURE

Fig. S2

ID

ZDB-FIG-180723-40

Publication

Enya et al., 2018 - A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.

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Fig. S2

Characterization of the pInt-Gal4-driven tumor model

(A) A gel image of genotyping of tumor-bearing larvae. Band sizes detecting Tg(5xUAS:EGFP-P2A-kras^G12D), Tg(pInt-Gal4) and tp53 are 701 bp, 345 bp and 88 bp, respectively. tp53 locus is used as a PCR control. M, DNA ladder marker: W, wild type larvae: G, parental Tg(pInt-Gal4) line: K, parental Tg(5xUAS:EGFP-P2A-kras^G12D) line. (B)-(I) Representative images of fluorescent immunohistochemistry for BrdU and EGFP in transversal sections of the posterior intestine of the sibling controls and tumor bearing larvae at 5 dpf. BrdU (B, C), Hoechst33342 (D, E) and EGFP (F, G) images are shown. In the merged images (H, I), BrdU, Hoechst33342 and EGFP signals are shown in red, blue and green, respectively. White arrows indicate intestinal cells positive for BrdU, Hoechst33342, and EGFP. Scale bar indicates 100 m. (J) The number of BrdU and EGFP positive intestinal cells. The number of BrdU and EGFP positive cells was counted from single section per individual larva. The data harbors 10 and 9 biological replicates from the sibling controls and tumor-bearing larvae, respectively. Error bars represent  s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.