FIGURE

Fig. 3

ID

ZDB-FIG-180710-30

Publication

Schorch et al., 2018 - Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL.

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Fig. 3

TpeL treatment reduces Ras-dependent hyperproliferation of melanocytes in vivo. (A) Micrographs of zebrafish larvae with Ras-dependent hyperpigmentation at 60 hpf treated with low (1 nM) and high (30 nM) concentrations of TpeL from 12 hpf on. A reduced pigmentation in the high TpeL concentration treated fish is seen over the whole larvae. The scale bar represents 0.1 mm. (B) TpeL- or untreated zebrafish larvae were pooled to 20 embryos per condition and lysed. Subsequently, Ras_non-gluc., pMEK, pERK, tERK, and tubulin were probed by immunoblotting. A representative blot of three independent experiments is shown. (C) Statistical analysis of phosphorylated MEK and ERK following treatment with TpeL as presented in B.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hzm1Et; io006Tg
Condition:	chemical treatment by environment: bacterial metabolite
Observed In:	melanocyte whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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