FIGURE

Fig. 3

ID

ZDB-FIG-180706-8

Publication

Siddam et al., 2018 - The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development

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Fig. 3

Celf1 deficiency in mouse and fish causes fiber cell nuclear degradation defects.

(A, B) Histological analysis of control and Celf1^{lacZKI/lacZKI} mouse lenses at post natal day 4 (P4) stage shows abnormal presence of nuclei in centrally located fiber cells only in Celf1^{lacZKI/lacZKI} mice. (A’, B’) High-magnification of the dotted-line area in A, B. Asterisk denote abnormally retained nuclei. (C, D) In zebrafish, compared to control, celf1^KD lens exhibit abnormal presence of nuclei in the central fiber cell region. (C’, D’) High-magnification of the dotted-line area in E, F. Asterisk denote abnormally retained nuclei. (E) RT-qPCR analysis confirms significant Dnase2b down-regulation in Celf1^cKO/lacZKI lenses compared to control. (F) RNA immunoprecipitation (RIP) and (G) cross-linked RNA immunoprecipitation (CLIP) shows Dnase2b to be enriched in Celf1-pulldown in wild-type mouse lens. (H) Celf1 over-expression in NIH3T3 cells, which carry Dnase2b 3’UTR downstream of a luciferase reporter, results in significant increase of luciferase mRNA. Abbr.: f.c., fold-change; NS, not significant. Asterisks in E, G, H indicate a p-value < 0.005.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-celf1
Knockdown Reagent:	MO3-celf1
Observed In:	lens fiber cell nucleus lens fiber cell differentiation
Stage:	Day 4

Phenotype Detail

Acknowledgments

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