Fig. 5

shank3b ^−/− zebrafish displayed social interaction defect. a–b Schematic of shoaling test (a) and significantly increased inter-fish distance of adult male shank3b^−/− zebrafish (3.5 mpf) (b). N = 18 for each group. c–f Schematic of social preference test of adult male zebrafish (3.5 mpf) (c). Heat map (d) shows that shank3b ^+/+ zebrafish displayed significant higher frequency near a group of zebrafish than shank3b ^−/− zebrafish. Time ratio (e) and distance ratio (f) in the conspecific sector were significantly reduced in shank3b ^−/− zebrafish compared to shank3b ^+/+ zebrafish. N = 16 for each group. g–h Schematic of kin recognition and preference test of adult male zebrafish (3.5 mpf) (g) and significantly reduced ratio of kin zone entering in shank3b ^−/− zebrafish compared to shank3 ^+/+ zebrafish (h). N = 10 for each group. Data are presented as mean ± SEM; ****p < 0.0001