FIGURE

Fig. S3

ID

ZDB-FIG-180705-18

Publication

Liu et al., 2018 - Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

lpl mutants develop hyperlipidemia. (A) Primers, flanking exon 4, the site of CRISPR target, are used to amplify lpl cDNA. (B) Single lpl cDNA band of the same size in WT and lpl mutant embryos indicates that no alternative splicing occurs in the mutant. (C) Sequence results indicate a 2nt deletion in cDNA from lpl mutants. (D) ORO staining of WT, apoc2 and lpl mutants at 6 dpf. (E) Representative images and quantitative results of BODIPY neutral lipid staining of WT, apoc2 and lpl mutants at 6 dpf. Results are mean±SEM; n=5 (WT and apoc2 mut) and n=6 (lpl mut); ***P<0.001 (Student’s t-test). Scale bars, 100 μm in D, and 50 μm in E.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apoc2^sd38/sd38 (AB) lpla^sd51/sd51 (AB)
Observed In:	whole organism lipid
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.