FIGURE

Fig. 6

ID

ZDB-FIG-180702-4

Publication

Hocking et al., 2018 - Morphogenetic defects underlie Superior Coloboma, a newly identified closure disorder of the dorsal eye

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Fig. 6

Analysis of Tbx2b and closure of the superior ocular sulcus.

(A) Whole-mount in situ hybridization for zebrafish tbx2b in control and BMP-depleted embryos. Top panels are eyes dissected from control and DMH1-treated embryos; bottom panels are from gdf6a^+/+, and gdf6a^-/- embryos. (B-C) Analysis of SOS closure in Tbx2b-depleted embryos. DIC images of eyes from live tbx2b^+/+ (top panel) and tbx2b^fby (bottom panel) embryos (B). Quantification of SOS closure in wild type and tbx2b^fby mutant zebrafish eyes (C). Data are means ± SEM; one-way ANOVA with Tukey’s test: *P<0.05. Scale bars are 50 μm.

Expression Data

Gene:	tbx2b
Fish:	WT gdf6a^s327/s327
Condition:	chemical treatment by environment: DMH1
Anatomical Term:	eye
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT gdf6a^s327/s327 tbx2b^c144/c144
Condition:	chemical treatment by environment: DMH1
Observed In:	eye superior ocular sulcus
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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