FIGURE

Fig. 3

ID

ZDB-FIG-180611-36

Publication

Colombo et al., 2017 - nkx genes establish SHF cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression

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Fig. 3

Nkx genes regulate OFT progenitor proliferation timing and differentiation. (A-D) Ventral view of the arterial pole region at 40 hpf. Confocal projections of immunohistochemistry for EdU (red), ZsYellow (green) and MF20 (gray) in embryos carrying Tg(nkx2.5:ZsYellow) following EdU incubation at 18 hpf in wild-type (A,B) and nkx2.5^−/−;nkx2.7^+/+ (C,D) embryos. White arrows indicate EdU⁺ ZsYellow⁺ cells. The boxed areas in A,C are shown at higher magnification in B,D. (E-G) Proliferation indices showing a statistically significant increase in undifferentiated, ZsYellow⁺ MF20⁻ progenitors in nkx mutant (n=6) compared with wild-type (n=6) embryos (G); the proliferation index is comparable in the proximal, ZsYellow⁺ MF20⁺ CM population contributing to the arterial pole (F) as well as in the total OFT, ZsYellow⁺ MF20^+/− CM population (E). Student's t-test was used to determine statistical significance. The mean and s.e.m. of each data set are shown (*P<0.005).

Expression Data

Gene:	ZsYellow
Antibody:	Ab-MF20
Fish:	fb7Tg nkx2.5^vu179/vu179; fb7Tg
Anatomical Term:	heart tube
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx2.5^vu179/vu179; fb7Tg
Observed In:	cardiac muscle myoblast cell population proliferation
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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