Fig. S1

(A) Lateral view of zebrafish embryos at 48 hpf injected either with an ATG-start morpholino against smyd1a (MO2-smyd1a) or a 5 bp mismatch morpholino as the respective control (Ctrl-MO2). (B) Quantification and statistical analysis of affected embryos showing pericardial edemaafter injection of the corresponding morpholinos. Statistical analysis of five independent experiments was performed using one-way ANOVA (****p<0.0001). Data are mean values +- SD. (C) For the generation of CRISPR/Cas9-mediated mutants (crispants) Cas9-Protein in complex with tracrRNA and smyd1a-targeting crRNA was injected into one-cell stage embryos and compared to a non-targeting gRNA (Ctrl-gRNA). Shown are lateral views of embryos at 48 hpf. (D) Lateral view of the rescued morphant phenotype (96 hpf) by injecting smyd1a-mRNA and MO1-smyd1a together in comparison to MO1-smyd1a + KCl injected embryos. (E) Quantification of affected embryos with heart and swim defects of smyd1a morphants after injection of MO2-smyd1a + KCl or together with smyd1a-mRNA at 96 hpf. The 5 bp mismatch morpholino (Ctrl-MO2) served as negative control. Shown are mean values of 4 independent experiments (+- SD) and statistical analysis was performed as in C (**** p<0.0001). (F) Quantification of the spontaneous movement assay of Ctrl-MO1/2 and MO1/2-smyd1a injected embryos. (G) Spontaneous movement assay with false-coloured superimposed overviews of 24 hpf control (Ctrl-MO1) injected and MO1-smyd1a injected embryos. Red: t = 0 sec; green: t = 5 sec.