FIGURE

Fig. 8

ID

ZDB-FIG-180524-20

Publication

Sánchez-Iranzo et al., 2018 - Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Trabecular tbx5a-derived cardiomyocytes within the cortical myocardium express the cortical marker Xirp2a. Immunofluorescence with anti-Xirp2a and GFP and anti-Myosin Heavy Chain (MHC) on ventricle sections. a–f Uninjured adult tbx5a:GFP ventricle. Xirp2a expression was observed in the cortical layer but not in the trabecular layer showing a complementary pattern with tbx5a:GFP as predicted by the RNA-Seq (n = 3/3). g–r Double transgenic tbx5a:mCherry-p2a-CreER^T2;ubb:loxP-lacZ-loxP-GFP were treated with 4-Hydroxytamoxifen (4-OHT) from 84 to 72 and 60 to 48 hours before cryoinjury. Hearts were fixed at 90 days postinjury (dpi). GFP⁺ cells marking the tbx5a lineage within the cortical layer were positive for Xirp2a, whereas GFP⁺ cells within the trabecular layer did not express this marker (n = 6/6). at, atrium; ba, bulbus arteriosus; v, ventricle. Scale bars, a, g 100 µm, c, m 25 μm, and h, n 10 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.