Fig. S2
Transgenic zebrafish (Gateways) expresses GFP in the AP, OVLT, SFO, ME, PVO, and migratory microglia. (A) anti-GFP WISH and (B) immunohistochemistry. (A,B) wholemount lateral view (eyes removed); anterior to the left. ah, adenohypophysis; ap, area postrema; c, cerebellum; cpIII, choroid plexus of III ventricle; cpIV, choroid plexus of IV ventricle; d, diencephalon; e, epiphysis; h, hour postfertilization; hb, hindbrain; ht, hypothalamus; mhb, midbrain-hindbrain boundary; me, median eminence; ot, optic tectum; ovlt, organum vasculosum laminae terminalis; pt, posterior tuberculum; pvo, paraventricular organ; r5, rhombomere 5; t, telencephalon, #, SFO. Scale bar 50–μm. *Defines clusters of cells expressing GFP mRNA. |