FIGURE

Fig. 3

ID

ZDB-FIG-180516-5

Publication

Zhang et al., 2017 - Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Excessive apoptotic early T lymphocytes in the thymus of rb1^smu8/smu8 mutants. (A) Double staining of BrdU/rag2-dsRed show BrdU incorporation of T cells in 4 dpf siblings and rb1^smu8/smu8 mutants (left). The white ovals indicate the thymus region. The white arrows indicate proliferative T cells. Scale bars: 20 μm. The graph shows the percentages of rag2:DsRed⁺ T cells that incorporate BrdU (mean± s.e.m.; ns, not significant; n=10). (B) Double staining of TUNEL/rag2-dsRed shows TUNEL incorporation by T-cells in 4 dpf siblings and rb1^smu8/smu8 mutants. The broken line outlines the thymus region. The white arrows indicate apoptotic T cells. Scale bar: 50 μm. The graph shows the percentages of rag2:DsRed⁺ T cells that incorporate TUNEL (mean±s.e.m.; ***P<0.001; n=9). (C) Confocal images of T cells (red) exhibiting cell apoptosis (overlap of TUNEL staining) of siblings and rb1^smu8/smu8 mutant embryos injected with control or pTol-lck:rb1 plasmid at 5 dpf. Scale bars: 50 μm. The graph shows the percentages of T cells (red) exhibiting cell apoptosis (overlap of TUNEL staining) in siblings and rb1^smu8/smu8 mutant embryos injected with control or pTol-lck:rb1 plasmid (mean±s.e.m.; ***P<0.001; n=10).

Expression Data

Gene:	DsRed
Fish:	zf411Tg rb1^sm11/sm11; zf411Tg
Anatomical Term:	thymus T cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rb1^sm11/sm11; zf411Tg
Observed In:	T cell apoptotic process
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development