FIGURE

Fig. 1

ID

ZDB-FIG-180503-1

Publication

Pelliccia et al., 2017 - Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning

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Fig. 1

Mutations in maternal gdf3 lead to defects in embryonic patterning.

(A) RNA in situ hybridization shows gdf3 mRNA is broadly expressed throughout early zebrafish development, followed by specific expression in Kupffer’s vesicle (arrow) and the lateral plate mesoderm (arrowhead). Views are indicated. (B) Schematic of gdf3 mutant alleles predicted to form truncated proteins due to early stop codons in the first exon. Purple regions indicate changes in amino acid sequence prior to the premature stop codon in each allele (see Material and Methods for more details). (C–F) Loss of maternally deposited Gdf3 causes patterning defects. Embryos heterozygous (C) or homozygous (D) for the pr05 allele appeared normal at 26 hpf. Heterozygous embryos from homozygous mothers (Mgdf) exhibited defects in mesoderm and endoderm formation (E). Maternal-zygotic gdf3 embryos (MZgdf3) had similar mesoderm and endoderm defects as Mgdf3 embryos (F). gdf3 mRNA injected at the one cell stage rescued defects in both Mgdf3 (G) and MZgdf3 (H) embryos. C-H are lateral views.

Expression Data

Gene:	gdf3
Fish:	WT
Anatomical Terms:	Kupffer's vesicle lateral plate mesoderm whole organism
Stage Range:	2-cell to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gdf3^pr05/pr05 gdf3^pr05/pr05 gdf3^pr05
Observed In:	endoderm formation mesoderm formation whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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