FIGURE

Fig. 5

ID

ZDB-FIG-180420-31

Publication

Dogra et al., 2017 - Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair

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Fig. 5

mstnb and inhbaa inversely regulate the activities of Smad2 and Smad3 response elements, as well as Smad3 phosphorylation. a RT-qPCR analysis for relative EGFP mRNA expression in 48 hpf Tg(ARE:EGFP) embryos injected with mstnb, inhbaa, mstnb-2A-H2B-mcherry, and inhbaa-2A-H2B-mcherry mRNA compared to RFP mRNA injected (n = 2 × 10 embryos assessed as 2 biological and 2 technical replicates). b RT-qPCR analysis for relative EGFP mRNA expression in 48 hpf Tg(12XSBE:EGFP) embryos injected with mstnb, inhbaa, mstnb-2A-H2B-mcherry, and inhbaa-2A-H2B-mcherry mRNA compared to RFP mRNA injected (n = 2 × 10 embryos assessed as two biological and two technical replicates). c, d Sections of wild-type sibling and mstnb OE cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). White dotted regions delineate the injured area. White arrowheads point to pSmad3⁺ CMs near the injured area. e Quantification of pSmad3⁺ CMs in wild-type sibling (n = 4) and mstnb OE (n = 5) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. f, g Sections of wild-type sibling and inhbaa OE cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). h Quantification of pSmad3⁺ CMs in wild-type sibling (n = 3) and inhbaa OE (n = 4) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. i, j Sections of mstnb ^+/+ and mstnb ^−/− cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). k Quantification of pSmad3⁺ CMs in mstnb ^+/+ (n = 4) and mstnb ^−/− (n = 4) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. l, m Sections of inhbaa ^+/+ and inhbaa ^−/− cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). n Quantification of pSmad3⁺ CMs in inhbaa ^+/+ (n = 4) and inhbaa ^−/− (n = 5) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. All cell counts were performed on three sections from each heart. Each data point on dot plot represents one heart (data are mean ± s.e.m., *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001—Student’s t test, two-tailed). Scale bars, 100 µm

Expression Data

Antibodies:	Ab-MF20 Ab2-smad3
Fish:	WT inhbaa^bns37/bns37 mstnb^bns5/bns5 bns145Tg bns146Tg
Condition:	cold damage: heart
Anatomical Terms:	cardiac muscle myoblast myocardium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	inhbaa^bns37/bns37 mstnb^bns5/bns5 bns145Tg bns146Tg
Condition:	cold damage: heart
Observed In:	cardiac muscle myoblast heart cardiac muscle cell proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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