FIGURE

Fig. 4

ID

ZDB-FIG-180418-19

Publication

Sarmah et al., 2017 - Embryonic Ethanol Exposure Affects Early- and Late-Added Cardiac Precursors and Produces Long-Lasting Heart Chamber Defects in Zebrafish

Other Figures

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Fig. 4

Embryonic ethanol exposure reduced second heart field precursors. (A–I) Anti-Islet1 antibody stained Tg(myl7:GFP) embryos showed Islet1 positive second heart field precursors in the rotating heart cone in control embryos (A–C) at 22 hpf; ethanol exposed embryos showed reduced number of Islet1 positive cells in the heart cones (D–I) at 22 hpf; (J–R) Anti-Islet1 antibody stained Tg(myl7:GFP) embryos showed second heart field derived cardiomyoctes in the heart (Myl7 and Islet1 double positive; red and green) and second heart field precursors (Islet1 positive, red) near the out flow pole in the control embryos (J–L) and ethanol-exposed embryos (M–R) at 28 hpf; (S) Graph shows the quantification of the Islet1/Myl7 double positive cardiomyocytes in the heart tube and Islet1 positive cells near the outflow pole at 28 hpf.

Expression Data

Gene:	EGFP
Antibody:	Ab2-isl
Fish:	twu34Tg
Condition:	chemical treatment by environment: ethanol
Anatomical Term:	cardiac muscle cell
Stage Range:	26+ somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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