FIGURE

Fig. 7

ID

ZDB-FIG-180320-10

Publication

Suarez-Bregua et al., 2017 - Targeted Pth4-expressing cell ablation impairs skeletal mineralization in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Whole mount in situ hybridization shows the gene expression pattern of osteoblast differentiation markers at 3 dpf in control (A, C and E) and ablated larvae (B, D and F) from lateral view.

Expression of sp7 is detected in operculum, cleithrum, teeth and parasphenoid (A). Note the absence of expression in cleithrum and operculum in ablated larvae (B). runx2b and dlx2b display the same expression pattern before ablation (C, E) and after ablation (D, F). Quantitative RT-PCR analysis of early (runx2b), intermediate (sp7) and late (sparc) stage markers of osteoblast differentiation in 7 dpf ablated (black bars) and control larvae (white bars) (G). Results show significant down-regulation of sparc gene expression (*p <0.05) when compared with controls. Results normalized to actb1 are expressed as mean ± SEM. Abbreviations: ps, parasphenoid; op, operculum; cb5, ceratobranchial arch 5; t, teeth; cl, cleithrum. Scale bars: 100 μm.

Expression Data

Genes:	dlx2b runx2b sp7 sparc
Fish:	iim07Tg; zf1028Tg
Condition:	ablation: neuron
Anatomical Terms:	cleithrum dental epithelium opercular flap osteoblast whole organism
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	iim07Tg; zf1028Tg
Condition:	ablation: neuron
Observed In:	cleithrum opercular flap whole organism
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One