FIGURE

Fig. S7

ID

ZDB-FIG-180305-7

Publication

Giannelou et al., 2018 - Aberrant tRNA processing causes an autoinflammatory syndrome responsive to TNF inhibitors

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Viability and phenotype of trnt1 knockout zebrafish embryos
A. Survival of trnt1-/- fish. The histogram shows the percentage of live fish with homozygous WT, heterozygous, and homozygous null genotypes for both alleles [knockout mutant alleles del10 (Δ10) and del13 (Δ13)] at the indicated days post fertilization (dpf). Null fish die between 6 to 9 dpf and no null adults are recovered from heterozygous in-crosses. B-E. Defective morphological and histological phenotypes of trnt1-/- embryos at 5 dpf. WT embryos are shown in the left panel and mutant embryos are shown in the right panel. B. trnt1-/- embryos with abnormal jaw (black arrow), pericardial edema (red arrow) and uninflated swim bladder (blue arrow). C-D. Abnormal pharyngeal arches shown by histology analysis (C, black arrow) and cartilage staining (D) E. Abnormal intestinal development. Mutant embryos show lack of epithelial folds in the bulb of intestine. Black arrows mark the indicated structures. Both alleles had similar phenotypes, phenotype for del10 allele is shown here.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	trnt1^hg38/hg38 trnt1^hg39/hg39
Observed In:	digestive tract development embryo development mandibular arch skeleton pericardium pharyngeal arch swim bladder whole organism
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ann. Rheum. Dis.