Fig. S3

Activation of distinct glomeruli by nucleotides, amino acids and, bile acids. Related to Figure2. (A) pERK immunostaining of whole-mount OB. Zebrafish were exposed to nucleotide mixture (ATP, IMP, TTP, 10 mM each), amino acid mixture (alanine, cysteine, histidine, lysine, methionine, phenylalanine, tryptophan, valine, 10 mM each), and bile acid mixture (glycocholic acid, taurocholic acid, taurodeoxycholic acid, 10 mM each) and their brains were subjected to immunohistochemistry with anti-pERK antibody. Amino acid mixture activates multiple glomeruli in the lateral cluster (lGx), while bile acid mixture activates dorsal and ventromedial glomerular clusters (dG and vmG). lG2 (arrowheads) is activated by nucleotide mixture, but not by amino acid and bile acid mixtures. Scale bars, 100 μm. (B) Ca2+ imaging of OB glomeruli in OMP:Gal4FF;SAGFF27A;UAS:G-CaMP7 transgenic zebrafish. Representative images of Ca2+ responses in lG2 and lGx upon stimulation with ATP, adenosine and alanine, respectively. Scale bar, 50 μm