FIGURE

Fig. S2

ID

ZDB-FIG-180209-9

Publication

Botthof et al., 2017 - Loss of the homologous recombination gene rad51 leads to Fanconi anemia-like symptoms in zebrafish.

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Fig. S2

Loss of rad51 causes DNA damage sensitivity. (A) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 5 μg/ml mitomycin C for 20 hours. White arrows indicate premature chromatid separation events (i). Quantification of the damage (ii). Mann- Whitney test, P=.001, n_+/+=16, n_-/-=19. (B) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 1 nM CPT for 20 hours. White arrows indicate breaks and radial structures in response to topoisomerase inhibition (i). Quantification of the damage (ii). Mann-Whitney test, P<.0001, n_+/+=12, n_-/-=17. (C) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 10μ μM DiQ for 20 hours (i). Quantification of the damage (ii). Mann-Whitney test, P=.13, n_+/+=14, n_-/-=29. Bars represent mean +/- SEM in all graphs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA