FIGURE

Fig. 6

ID

ZDB-FIG-180207-8

Publication

Choi et al., 2018 - Targeted knockout of a chemokine-like gene increases anxiety and fear responses

Other Figures

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Fig. 6

Increase of mRNA expression of stress-related crhb in sam2^cnu1 KO fish and spontaneous inhibitory postsynaptic currents onto CRH neurons by SAM2. (A–D) Increase of crhb mRNA expression in sam2^cnu1 KO fish (sam2^+/+, n = 6; sam2^−/−, n = 6). Ventral views of the whole brain of control sam2^+/+ (A and C) and sam2^−/− KO fish (B and D). (A′ and B′) Higher magnifications of the boxed regions in A and B are the PPa in zebrafish, homologous to the mouse PVN. (Scale bars, 200 µm.) (E) Representative photomicrograph of the Cre-dependent TdTomato in the PVN CRH neurons. (Magnification: E, 10×.) (F) Representative voltage-clamp traces of sIPSCs in response to SAM2 application in PVN CRH neurons. (G) SAM2 application significantly increased sIPSC frequency. (H) SAM2 did not affect sIPSC amplitude. (I) Representative voltage-clamp trace of eIPSC onto CRH neurons in response to SAM2 application. (J) SAM2 did not affect the amplitude of eIPSCs. (K) SAM2 did not change the paired-pulse ratio. *P < 0.05; **P < 0.01.

Expression Data

Gene:	crhb
Fish:	WT tafa2^{ck001a/ck001a}
Condition:	stress
Anatomical Term:	hypothalamus preoptic area
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tafa2^{ck001a/ck001a}
Condition:	stress
Observed In:	hypothalamus preoptic area
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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