FIGURE

Fig. S15

ID

ZDB-FIG-180206-13

Publication

Singh et al., 2017 - Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth

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Fig. S15

Pharmacological inhibition of Notch and NF-kB signaling increases the number of recently-differentiated cells on the islet’s anterior side. (A) Tg(ins:H2B-GFP);Tg(ins:DsRed) animals were treated with DMSO (control), a γ-secretase inhibitor (LY-411575) or an NF-kB inhibitor (Ethyl Pyruvate (EP)), and the number of recently-differentiated beta-cells was quantified. The recently-differentiated beta-cells are H2B-GFP-positive but DsRed-negative. Maximum intensity projections of 18 dpf primary islets. The anterior side is to the top. (B) Quantification of recently-differentiated beta-cells within the anterior and posterior halves of the primary islet (n = 20 islets) for controls and animals treated with LY-411575 or EP. The plot shows tukey style boxplot overlaid with the data points. In all three cases, the number of recently-differentiated beta-cells was higher within the anterior compared to the posterior half (two-tailed t-test, *** p < 0.001). Treatment with LY-411575 or EP significantly enhanced (two-tailed t-test, *** p < 0.001) the addition of beta-cells within the anterior halves of the islets, as compared to controls, but not within the posterior. Scale bars, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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