FIGURE

Fig. S7

ID

ZDB-FIG-180126-47

Publication

Ma et al., 2017 - A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Supplementary information for line pIDM-E10. (A) Reduction of the grb14 expression in the pIDM-E10 embryos treated with Dox. The treated embryos were sampled at 24 hpf and subjected to WISH. Anti-sense RNA of grb14 was used to perform WISH. Number, type of represented embryos/total embryos in F₂ population. (B) Induction of the anti-sense transcript of grb14 in the pIDM-E10 embryos treated with Dox was analyzed with qRT-PCR using the primer pairs grb14-intron2-F and grb14-intron2-R. (C) RT-PCR analysis showed that, due to abnormal splicing, the grb14 morpholino (grb14-MO) had created an aberrant transcript with the extra 386 nt. WT embryos injected with the standard control morpholino (SC-MO) were used as the control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.