FIGURE

Fig. 2

ID

ZDB-FIG-180124-20

Publication

Shen et al., 2017 - Ikk2 regulates cytokinesis during vertebrate development

Other Figures

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Fig. 2

Loss of Ikk2 caused hemorrhage. (A,B) Bright-field images of wild-type and ikk2 ^−/− (zygotic) embryos obtained from heterozygotic parents at 48 hpf. Red arrows - the hemorrhage site. (A,A′) Ventral view. (B,B′) dorsal view. (C) Quantification of hemorrhaging embryos. (D) Quantification of embryos with cranial hemorrhage vs that in other parts of the body. (C,D) Unpaired Student’s t test. (E,F) Confocal Z-projection shows wild type (E) and ikk2 ^−/− (F) embryos of the Tg(fli:EGFP)/Tg(gata:DsRed) background at 48 hpf. (E′,F′) Confocal Z-projection shows Tg(fli:EGFP) embryos without the dorsal most MtA, MsA and DLV. EGFP was pseudo-colored as black. PHBC (blue), BA (red), and CtAs (purple) were marked. * - the affected CtAs with cranial hemorrhage in (F). All data expressed as mean ± SEM; scale bar in all panels: 100 μm. Abbreviations – PHBC: primordial hindbrain channels; BA: basilar artery; CtAs: central arteries (51).

Expression Data

Genes:	DsRed EGFP
Fish:	sd2Tg; y1Tg ikbkb^sq302/sq302; sd2Tg; y1Tg
Anatomical Terms:	basilar artery central artery endothelial cell nucleate erythrocyte primordial hindbrain channel
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ikbkb^sq302/sq302 ikbkb^sq302/sq302; sd2Tg; y1Tg
Observed In:	central artery head
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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