Fig. 3
- ID
- ZDB-FIG-180103-15
- Publication
- Sidhaye et al., 2017 - Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis
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Rim involution involves active cell migration of connected epithelial cells. (A) Time-lapse imaging of rim zone with mosaic expression of Par3-GFP and mKate2-ras. Arrows show membrane protrusions. Frames from Video 4. N = 5. (B) Time-lapse imaging of rim zone with mosaic expression of GFP-UtrophinCH and ras-mKate2. Inlays show zoomed marked area. Arrows show actin localization in the protrusions. Arrowheads mark the basally enriched stable actin pool in the RNE. Frames from Video 5. N = 6. (C) Time-lapse imaging of rim zone with mosaic expression of GFP-UtrophinCH and ras-mKate2. Yellow arrows show apical actin localization at adherens junctions. Frames from Video 7. N = 6. (D) Schematic of a rim cell exhibiting actin based protrusions at the basal side. The arrow marks the direction of involution. The leading and lagging edges indicate the sides referred in (E) and (F). (E) Number of actin protrusions observed per hour in rim cells in the control condition. Each pair of datapoints represents two sides of the same rim cell. n = 9, N = 6 . See Figure 3—source data 1. (F) Number of actin protrusions observed per hour in the rim cells in Rockout treatment condition. Each pair of datapoints represents two sides of the same rim cell. n = 6, N = 6. See Figure 3—source data 1. (G) Confocal scan of rim zone in Rockout-treated embryos with mosaic expression of GFP-UtrophinCH and ras-mKate2. Inlays show enlarged marked area. Arrows show actin localization in the protrusions. Frames from Video 6. N = 6 all scale bars = 10 µm. All movies started around 17 ss -18 ss, Time in h:min. |