FIGURE

Fig. 1

ID

ZDB-FIG-171227-3

Publication

Shao et al., 2017 - Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

VAb@2cell inhibits BMP signalling. (A,B) Decreased P-Smad1/5/8 immunostaining (green), merged with DAPI (blue), in VAb@2cell embryos at 60% epiboly. (C,D) Analysis by western blotting of P-Smad1/5/8 levels in VAb@2cell and VAb@1cell embryos at 70% epiboly. P-Smad1/5/8 levels are normalised to β-actin, and BMP activity in intact embryos is set as 1. Data are mean±s.d. from three independent experiments. (E-G) Analysis by in situ hybridisation of indicated genes. Animal pole views with dorsal at the top. (H) Procedure of cell transplantation to visualise cell non-autonomous inhibition of BMP signalling in VAb@2cell embryos using P-Smad1/5/8 immunofluorescence (IF) staining. (I) RLDx-labelled donor cells transplanted at different locations of the margin in unlabelled intact embryos at 70% epiboly. (J-O) Similar presence of P-Smad1/5/8 immunostaining in transplanted cells derived from intact (J-L) or VAb@2cell (M-O) embryos. Scale bars: 250 µm in A,B,E-G; 20 µm in J-O.

Expression Data

Genes:	eve1 gata2a gsc tbxta
Antibody:	Ab1-smad
Fish:	WT
Condition:	ablation: yolk
Anatomical Terms:	anatomical structure whole organism
Stage Range:	Germ-ring to Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	ablation: yolk
Observed In:	anatomical structure BMP signaling pathway whole organism
Stage Range:	50%-epiboly to Shield

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development