FIGURE

Fig. S5

ID

ZDB-FIG-171127-15

Publication

Sedykh et al., 2016 - Novel roles for the radial spoke head protein 9 in neural and neurosensory cilia

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Morpholino knockdown of Rsph9 causes structural axonemal defects.

A-H: TEM images of ciliary cross-sections in rsph9 morphant embryos at 1 dpf. Ventral neural cilia with 9+0 axonemes found in the ventral midbrain (A) and spinal cord (B) of uninjected control embryos. Note outer dynein arms (arrow), indicative of ciliary motility. Rsph9-depleted morphants contain aberrant 8+1 cilia in the ventral midbrain (C, D). Pronephric cilia in uninjected controls have 9+2 axonemes (E). Rsph9 morphants contain a mixture of cilia with 9+2 (F), supernumerary central microtubules, e.g. 9+5 (G) and 9+0 (H). I: Summary of TEM analysis of ciliary axonemes in wildtype, control morpholino-injected, and Rsph9 morpholino-injected embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rsph9
Knockdown Reagent:	MO3-rsph9
Observed In:	neural tube axoneme pronephric duct axoneme
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.