FIGURE

Fig. 8

ID

ZDB-FIG-171122-10

Publication

Chou et al., 2016 - The endoderm indirectly influences morphogenetic movements of the zebrafish head kidney through the posterior cardinal vein and VegfC

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Fig. 8

Both vegfCMO and flt4MO rescue the convergence of the IR in sox32-deficient embryos.

(A) (Upper) Colocalization of vegfC with wt1b and ff1b, respectively, in the 25 hpf embryo by ISH. (Lower) A cross-section of a 34 hpf embryo showing that VegfC protein (red) was enriched in the kidney (delineated by wt1b:GFP). (B) sox32MO, vegfCMO, flt4MO, sox32/vegfC double-MOs, sox32/flt4 double-MOs were injected into Tg(kdrl:EGFP)^s843 embryos. The injected embryos were subjected to 3β-Hsd staining and analysed for the effects of MOs on the morphology of the IR (delineated by the 3β-Hsd activity and marked by orange arrows) and the axial vasculature (delineated by kdrl:EGFP). Dorsal views, with anterior to the top. Distances between bilateral IRs and between the bilateral edges of the PCV at the level of the IR (marked by red brackets) are quantified in (C). (D) Dorsolateral views of the control, vegfCMO-injected, and flt4MO-injected Tg(kdrl:EGFP)^s843 embryos at 34 hpf, with anterior to the left, to show the relative distribution of the DA, PCV and IR. Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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