Fig. 1

Combined loss of Neurog1 and Neurod4 function disrupts habenular neurogenesis.

Whole-mount in situ hybridization against cxcr4b (A-D) or brn3a (F-H) showing the epithalamus of wild type (A,E), neurog1^hi1059 mutant embryos (B,F), neurod4 morpholino injected embryos (C,G) or neurog1^hi1059;neurod4 morphant embryos (D,H) at 36 (A-D) or 48 (E-H) hpf. The expression of cxcr4b and brn3a appears unaffected in neurod4 morpholino injected embryos (C, n = 13/13 and G, n = 14/15) as compared with the expression in wild type (A, n = 9/9 and E, n = 9/9); cxcr4b and brn3a expression is also unaffected in neurog1^hi1059 mutant (B, n = 12/14 and F, n = 9/11) or only slightly decreased in few cases (respectively n = 2/14 and n = 2/11; data not shown). In contrast, the expression of both genes is either abrogated or strongly reduced in neurog1^hi1059;neurod4 morphants (D, n = 16/16 and H, n = 12/14). Embryos are viewed dorsally with anterior up.