FIGURE

Fig. 4

ID

ZDB-FIG-171117-14

Publication

Kamezaki et al., 2016 - Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Expression of N-CISSOR in zebrafish.

(a) Schematic representation of the procedure used for transgenesis and sample preparation. (b,c) Lateral view of the resulting F₁ Tg (HuC:Gal4-VP16; 5 × UAS:N-CISSOR) embryo at 36 hpf under a fluorescence stereomicroscope (b) and a confocal microscope (c), showing N-CISSOR expression in the nervous system. (b) Spinal motor nerves and posterior lateral line are indicated by the arrows and arrowhead, respectively. Scale bar: 200 μm. (c) Maximum projection of z-stack images and views of magnified areas surrounded by solid and dotted borders are shown. High colocalization of GFP and mCherry in somas (large asterisk) and low colocalization in axons (small asterisks). Intracellular (arrows) and extracellular (arrowheads) accumulation of mCherry. The mCherry/GFP fluorescence ratio image is shown in the right column. Scale bar: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.