Fig. 8

Cardiac developmental defects in mog1 morphants are rescued by overexpression of nkx2.5.

Zebrafish embryos were injected with Std-MO (16 ng; A,E), mog1 MO1 (16 ng; B,F), mog1 MO1 (16 ng) together with in vitro synthesized full-length zebrafish (C) or human MOG1 mRNA (200 pg) (D,G) and used for whole-mount in situ hybridization at 12 somites with antisense probes for nkx2.5 (A–D) or cmlc2 (E–G). Reduced expression of nkx2.5 by knockdown of mog1 was rescued by injection of mog1 mRNA (A–D). Co-injection of nkx2.5 mRNA (G) rescued the cardiac developmental defects in mog1 MO1 morphants (whole-mount in situ hybridization signal for cmlc2). (A–D) Dorsal view with the anterior at the top. (E–G) Anterior view with the ventral side at the top. The numbers in the upper right corner (e.g. 33/37 in A) describe the ratio of the number of embryos with the phenotype (e.g. 33) in the image over the number of total embryos analyzed (e.g. 37).