FIGURE

Fig. 7

ID

ZDB-FIG-171025-33

Publication

Panza et al., 2015 - The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm

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Fig. 7

vasna/vasnb double mutants do not display overt axon guidance defects at the optic chiasm. a Alleles generated by targeted mutagenesis of the vasna locus, using TALEN technology. All alleles are followed by premature stop codons, leading to the translation of a short and functionally inactive protein. Yellow highlights show the genomic DNA target sequence for the engineered nucleases. b vasna alleles generated by targeted mutagenesis display complete loss of Vasna protein in homozygosity. AC: anterior commissure. FP: floor plate. OC: optic chiasm. POC: postoptic commissure. Single optical sections from confocal Z-stacks. Lateral views. Anterior is to the left. Scale bar: 50 μm. c The vasnb ^sa6027 TILLING allele leads to complete loss of function, as suggested by the absence of Vasnb immunoreactivity. Single optical sections from confocal Z-stacks. Frontal views. Scale bar: 50 μm. d 5 dpf vasna/vasnb double mutant larvae do not display strong axon guidance phenotypes at the optic chiasm. Ventral views of anterogradely-labeled optic nerves at the optic chiasm. Anterior is to the top. Scale bar: 50 μm

Expression Data

Genes:	vasna vasnb
Antibody:	Ab1-tuba
Fish:	TU vasna^{zf1059/zf1059} vasnb^{sa6027/sa6027}
Anatomical Terms:	anterior commissure axon diencephalon mesenchyme diencephalon ventral region floor plate postoptic commissure axon retinal ganglion cell axon
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vasna^{zf1059/zf1059} vasnb^{sa6027/sa6027} vasnb^{sa6027/sa6027}; vasna^{zf1059/zf1059}
Observed In:	diencephalon mesenchyme diencephalon ventral region floor plate optic nerve formation optic nerve morphogenesis retinal ganglion cell axon guidance
Stage Range:	Prim-25 to Day 5

Phenotype Detail

Acknowledgments

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