FIGURE

Fig. 2

ID

ZDB-FIG-171024-15

Publication

Cambier et al., 2017 - Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages

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Fig. 2

Mycobacteria Mediate CCR2-Dependent Monocyte Recruitment by Actively Inducing CCL2 in Resident Macrophages

(A) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type or myeloid cell-deficient fish with 250–300 wild-type Mm.

(B–D) In situ hybridizations against zebrafish ccl2 mRNA following hindbrain ventricle infections with vehicle (bacterial media) (B), 80 wild-type Mm (C), 80 Mm - PGL− (D). Black arrows, ccl2 mRNA-positive phagocytes; white arrows ccl2 mRNA-negative phagocytes. Scale bar, 50μm. Results representative of three independent experiments.

(E) Mean brain resident macrophage numbers of csfr1^+/− and csfr1^−/− zebrafish at 2dpf. Results representative of two independent experiments.

(F) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1^+/− or csfr1^−/− fish after infection with 80 wild-type Mm.

(G) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type fish with 250–300 live or heat-killed wild-type Mm.

(H) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) wild-type Mm.

(I) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1^+/− or csfr1^−/− fish after infection with 80 Mm - PDIM⁻.

(J) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) Mm - PDIM⁻.

(K) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 wild-type or PDIM⁻ Mm.

Results in (F) and (H) through (K) representative of at least three independent experiments.

Expression Data

Gene:	ccl38a.5
Fish:	AB
Condition:	bacterial treatment by injection: Mycobacterium marinum
Anatomical Term:	fourth ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	csf1ra^{j4blue/j4blue} (AB)
Condition:	bacterial treatment by injection: Mycobacterium marinum
Observed In:	brain immune response in brain or nervous system brain macrophage fourth ventricle macrophage fourth ventricle monocyte
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Immunity, 47(3), Cambier, C.J., O'Leary, S.M., O'Sullivan, M.P., Keane, J., Ramakrishnan, L., Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages, 552-565.e4, Copyright (2017) with permission from Elsevier. Full text @ Immunity