FIGURE

Fig. 1

ID

ZDB-FIG-171018-56

Publication

Meng et al., 2017 - Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes

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Fig. 1

Long-term high-fat diet induced Type 2 Diabetes-like symptoms in zebrafish. Adult zebrafish were fed a normal diet (Ctrl) or a high-fat diet (HFD) for 10 weeks. (A)-(C) Comparison of morphology (A), body weight (B), and body mass index (BMI; weight/length, g/cm) (C) between Ctrl and HFD zebrafish. (D) Higher fasting blood glucose induced in the HFD group compared to the normal diet group. (E) Representative images of hematoxylin and eosin (H&E) staining show lipid vesicles in zebrafish liver slices in both groups. The histogram shows the average number of fat cavities per sight field in the control group and HFD group, which were counted from ten sight fields each group. (F) Representative electrophorogram of insulin bands from RT-PCR (left) and real-time qPCR results (right) show the mRNA levels of insulin gene transcription in the liver, brain, and muscle of zebrafish in both groups. (G) The mRNA levels of insulin receptor substrate-2 (IRS-2) and glucose transporter 2 (GLUT2) assayed by RT-PCR electrophorogram (left) and real-time qPCR results (right) in zebrafish liver. *p<0.05, **p<0.01, ***p<0.001 vs the control group.

Expression Data

Genes:	ins irs2a slc2a2
Fish:	AB
Condition:	high fat
Anatomical Terms:	brain liver muscle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	high fat
Observed In:	blood glucose brain liver liver lipid droplet muscle whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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