FIGURE

Fig. 3

ID

ZDB-FIG-171011-18

Publication

D'Agati et al., 2017 - A defect in the mitochondrial protein mpv17 underlies the transparent casper zebrafish

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Fig. 3

(A) Injection of full-length mpv17 mRNA leads to rescue of the roy orbison mutant phenotype, as demonstrated by the appearance of iridophores both in the eye and throughout the body of the fish (roy n = 0/20 fish with eye iridophores, roy+mRNA injection, n = 14/20 fish with eye iridophores). (B) ATG morpholino knockdown of mpv17 in the AB fish leads to a strong phenocopy of the roy phenotype, with loss of eye and body iridophores (AB = 71/79 fish with eye iridophores, AB+morpholino injection, n = 8/67 fish with eye iridophores). (C) Quantification of eye iridophores after morpholino injection to mpv17 shows a significant increase in # of fish with absent iridophores (*, p<0.05, paired t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mpv17^a9/a9 AB + MO1-mpv17
Knockdown Reagent:	MO1-mpv17
Observed In:	iridophore
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 430(1), D'Agati, G., Beltre, R., Sessa, A., Burger, A., Zhou, Y., Mosimann, C., White, R.M., A defect in the mitochondrial protein mpv17 underlies the transparent casper zebrafish, 11-17, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.